2008 Vol. 32, No. 4
Display Method:
2008, 32(4): 193-196.
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Abstract:
Objective To improve the stability of 11C-AC yields with CTI Inc, USA automatic synthesis module and to evaluate the product with biodistribution and imaging studies. Methods 1. Synthesis of 11C-AC. The amount of quenching water was fixed to 0.2 ml instead of fluctuating from 0.1 to 0.2 ml. The purifying and distilling temperature were raised to 140℃-145℃ instead of 135℃. Thin layer chromatography was performed on products for quality control. 2. Biodistribution studies. (1) Twenty female mice were divided into 4 groups. Each mouse was killed separately 0.5 min, 5 min, 10 min and 20 min after injection. Blood and organs were weighed and their radioactivity measured (calculated to ID%/g). (2) Two female mice were killed 0.5 min and 20 min separately after injection with 11C-AC and imaged, (3) Three female volunteers underwent PET studies after injection. Initial upper abdomen scan for 2 cases, delayed whole body scan for 3 cases and brain scan for 1 case were performed. Results (1) Synthesis of 11C-AC, A total of 113 syntheses were performed with original method. The yields of 61 syntheses were <370MBq (less than one clinical dose). After improvement, the yields of 17 syntheses were all >370 MBq (12 of them>740 MBq). The Rf of thin layer chromatograph was 0.7-0.8 and radiochemical purity>90%. (2) Biodistribution studies. The high uptake by heart and kidneys changed rapidly with time. Pancreas, liver and spleen had moderate uptake and clearance. (3) Imaging studies. In normal persons, heart, kidneys and pancreas showed high uptake in initial image and cleared rapidly from heart and kidneys, but the pancreas showed persistent high uptake in delayed image also. There was no radioactivity in bladder and brain. Conclusion The yield of 11C-AC syntheses by our improved method is stable and fulfills the requirement for clinical uses.
Objective To improve the stability of 11C-AC yields with CTI Inc, USA automatic synthesis module and to evaluate the product with biodistribution and imaging studies. Methods 1. Synthesis of 11C-AC. The amount of quenching water was fixed to 0.2 ml instead of fluctuating from 0.1 to 0.2 ml. The purifying and distilling temperature were raised to 140℃-145℃ instead of 135℃. Thin layer chromatography was performed on products for quality control. 2. Biodistribution studies. (1) Twenty female mice were divided into 4 groups. Each mouse was killed separately 0.5 min, 5 min, 10 min and 20 min after injection. Blood and organs were weighed and their radioactivity measured (calculated to ID%/g). (2) Two female mice were killed 0.5 min and 20 min separately after injection with 11C-AC and imaged, (3) Three female volunteers underwent PET studies after injection. Initial upper abdomen scan for 2 cases, delayed whole body scan for 3 cases and brain scan for 1 case were performed. Results (1) Synthesis of 11C-AC, A total of 113 syntheses were performed with original method. The yields of 61 syntheses were <370MBq (less than one clinical dose). After improvement, the yields of 17 syntheses were all >370 MBq (12 of them>740 MBq). The Rf of thin layer chromatograph was 0.7-0.8 and radiochemical purity>90%. (2) Biodistribution studies. The high uptake by heart and kidneys changed rapidly with time. Pancreas, liver and spleen had moderate uptake and clearance. (3) Imaging studies. In normal persons, heart, kidneys and pancreas showed high uptake in initial image and cleared rapidly from heart and kidneys, but the pancreas showed persistent high uptake in delayed image also. There was no radioactivity in bladder and brain. Conclusion The yield of 11C-AC syntheses by our improved method is stable and fulfills the requirement for clinical uses.
2008, 32(4): 197-201.
Abstract:
PET-CT is a successful combination of functional and morphologic information.and it has already been shown to have great value both in clinics and in scientific research.MRI is another kind of morphologic imaging method,in contrast to CT,MRI can yield images with higher sof t-tissue contrast and better spatial resolution.The combination of PET and MRI for simultaneous data acquisition should have far. reaching consequences for molecular imaging.This review will talk about the problems met in the development of PET-MRI and describe the progress to date and look forward to its potential application.
PET-CT is a successful combination of functional and morphologic information.and it has already been shown to have great value both in clinics and in scientific research.MRI is another kind of morphologic imaging method,in contrast to CT,MRI can yield images with higher sof t-tissue contrast and better spatial resolution.The combination of PET and MRI for simultaneous data acquisition should have far. reaching consequences for molecular imaging.This review will talk about the problems met in the development of PET-MRI and describe the progress to date and look forward to its potential application.
2008, 32(4): 202-205.
Abstract:
Along with the rapid development of PET and PET-CT technologies,the application of 18F-labelled radiopharmaeeuticals is more and more widespread in clinic.18F has predominant physical features with long half-life (110min).Also,the chemical nature of 18F is similar to that of hydrogen,it can't change the labelled molecular's character obviously.Therefore,the 18F is the best choice of radionuclide used in PET. However,natural animo acid has the character of metabolizing easily and complexly,the studies in non-natmal animo acid have increased gradually such as tyrosine derivatives,phenylalanine derivatives,praline derivatives,methionine derivatives,and so on.The article summarizes the 18F-labelled non.natural amino acids and their derivatives studied in recent years.
Along with the rapid development of PET and PET-CT technologies,the application of 18F-labelled radiopharmaeeuticals is more and more widespread in clinic.18F has predominant physical features with long half-life (110min).Also,the chemical nature of 18F is similar to that of hydrogen,it can't change the labelled molecular's character obviously.Therefore,the 18F is the best choice of radionuclide used in PET. However,natural animo acid has the character of metabolizing easily and complexly,the studies in non-natmal animo acid have increased gradually such as tyrosine derivatives,phenylalanine derivatives,praline derivatives,methionine derivatives,and so on.The article summarizes the 18F-labelled non.natural amino acids and their derivatives studied in recent years.
2008, 32(4): 206-208,256.
Abstract:
The new development and situation of targeted radionuclide therapy in oncology is described,which include radioimmunotherapy,peptide receptor radionuclide therapy,gene therapy and radionuclide labled chemotherapeuties therapy.The application research on labled carrier of those therapy is emphasized.Meanwhile,the research pmgess of indomethacin and its combined with targeted radionuclide therapy is also described.
The new development and situation of targeted radionuclide therapy in oncology is described,which include radioimmunotherapy,peptide receptor radionuclide therapy,gene therapy and radionuclide labled chemotherapeuties therapy.The application research on labled carrier of those therapy is emphasized.Meanwhile,the research pmgess of indomethacin and its combined with targeted radionuclide therapy is also described.
2008, 32(4): 209-213.
Abstract:
The risk for conversion to dementia is much higher in individuals with mild eoghitived impairmaent (MCI).The early detection of MCI individuals who will later convert to Alzheimer disease is an important issue for both clinical and research interests.Radionuclide imaging has been broadly used to study cerebral membolism and blood flow,as well as predicting the prognosis of MCI.This review summarizes the results of these studies assessing the predicting value of radionuelide imaging used alone and with other methods in recent years.
The risk for conversion to dementia is much higher in individuals with mild eoghitived impairmaent (MCI).The early detection of MCI individuals who will later convert to Alzheimer disease is an important issue for both clinical and research interests.Radionuclide imaging has been broadly used to study cerebral membolism and blood flow,as well as predicting the prognosis of MCI.This review summarizes the results of these studies assessing the predicting value of radionuelide imaging used alone and with other methods in recent years.
2008, 32(4): 214-216.
Abstract:
Objective To assess the use value of 18F-FDG PET-CT in non-small cell lung cancer. Metheds (1) The CT and PET-CT image of 32 cases non-small cell lung cancer caseswere compared in staging. (2) The relativety between tumor size and SUVmax was observed. (3) Thirty cases with accepted therapy were compared to find the differences of remains and new outbreak lesions.Results (1) Seven cases were upstaged and 7 cases were downstaged.Changing rate in staging by PET-CT Was 43.8% (14/32).The difference of altered stage lymph node and metastasis in number was not in statistical significance by 18F-FDG PET-CT. (2) Spearman grade correlation coefficient ra was 0.426.There were positive correlation between SUVmax and the tumor size (P<0.05). (3) PET-CT found 3 more remain lesions (Z=-0.520,P>0.05) and 19 mole new outbreak lesion (Z=-2.87 1,P<0.05) than that of CT.Conclusion 18F-FDG PET-CT had superiority in determining staging of non-small cell lung cancer,and was conducive in cheaking new outbreak lesions of accepted therapy eases.
Objective To assess the use value of 18F-FDG PET-CT in non-small cell lung cancer. Metheds (1) The CT and PET-CT image of 32 cases non-small cell lung cancer caseswere compared in staging. (2) The relativety between tumor size and SUVmax was observed. (3) Thirty cases with accepted therapy were compared to find the differences of remains and new outbreak lesions.Results (1) Seven cases were upstaged and 7 cases were downstaged.Changing rate in staging by PET-CT Was 43.8% (14/32).The difference of altered stage lymph node and metastasis in number was not in statistical significance by 18F-FDG PET-CT. (2) Spearman grade correlation coefficient ra was 0.426.There were positive correlation between SUVmax and the tumor size (P<0.05). (3) PET-CT found 3 more remain lesions (Z=-0.520,P>0.05) and 19 mole new outbreak lesion (Z=-2.87 1,P<0.05) than that of CT.Conclusion 18F-FDG PET-CT had superiority in determining staging of non-small cell lung cancer,and was conducive in cheaking new outbreak lesions of accepted therapy eases.
2008, 32(4): 218-220.
Abstract:
Objective To evaluate the value of plasma brain natriuretic peptide (BNP) level for differentiating eardiogenie dyspnea from pulmogenic dyspnea.Methods The detection of plasma BNP was carried out in 248 patients with dyspnea.The apparatus was the CENTUR type chemiluminescence detection produced by the Bayer companies of USA.All patients were divided into three groups depending on disease severity.The level of plasma BNP in each group wag compared.Results The level of plasma BNP in pulmonary disease complicated with left ventricular dysfunction was higher than that of pulmonary disease non-complicated with left ventricular dysfunction.There was significant difference between two groups (χ2=25.597,P<0.001).The level of plasma BNP in pulmonary disease complicated with right ventricular dysfunction was higher than that of simple pulmonary disease.There was no significant difference between two groups (t=1.614,P>0.05).The level of plasma BNP in pulmonary disease complicated with left ventricular dysfunction was higher than that of pulmonary disease complicated with fight ventricular dysfunction.There Was significant difference between two groups (t=2.53 l,P<0.05).If the left ventricular dysfunction degree was notable,the level of plasma BNP waft,high.There was also significant difference in different cardiac function classification by New York Heart Association (χ2=29.463,P<0.001).Conclusion Plasma BNP analysis is an impotent method for differentiating cardiogenic dyspnea from pulmogenie dyspnea.Meanwhile,it is also helpful to evaluating the severity of pulmonary disease complicated with left ventricular dysfunction.
Objective To evaluate the value of plasma brain natriuretic peptide (BNP) level for differentiating eardiogenie dyspnea from pulmogenic dyspnea.Methods The detection of plasma BNP was carried out in 248 patients with dyspnea.The apparatus was the CENTUR type chemiluminescence detection produced by the Bayer companies of USA.All patients were divided into three groups depending on disease severity.The level of plasma BNP in each group wag compared.Results The level of plasma BNP in pulmonary disease complicated with left ventricular dysfunction was higher than that of pulmonary disease non-complicated with left ventricular dysfunction.There was significant difference between two groups (χ2=25.597,P<0.001).The level of plasma BNP in pulmonary disease complicated with right ventricular dysfunction was higher than that of simple pulmonary disease.There was no significant difference between two groups (t=1.614,P>0.05).The level of plasma BNP in pulmonary disease complicated with left ventricular dysfunction was higher than that of pulmonary disease complicated with fight ventricular dysfunction.There Was significant difference between two groups (t=2.53 l,P<0.05).If the left ventricular dysfunction degree was notable,the level of plasma BNP waft,high.There was also significant difference in different cardiac function classification by New York Heart Association (χ2=29.463,P<0.001).Conclusion Plasma BNP analysis is an impotent method for differentiating cardiogenic dyspnea from pulmogenie dyspnea.Meanwhile,it is also helpful to evaluating the severity of pulmonary disease complicated with left ventricular dysfunction.
2008, 32(4): 221-223.
Abstract:
Objective To investigate the effect of different status of thyroid function (hypothyroidism and hyperthyroidism as well a8 euthyroid status)on serum ghrelin and leptin levels.Methods The levels of semm ghrelin and leptin were determined by radio immunoassay in 46 untreated subjects with hyperthyroidism.15 hyperthyroid patients achieved aeuthyroid status after radioiodine 131I therapy,21 cases of hypothyroidism and 18 cases of normal controls,respectively.Meanwhile,the serum levels of free triiodothyronine (FT3),free thyroxine (FT4) and thyroid-stimulating hormone (TSH) were measured by chemiluminescence immune assay.Results (1) The levels of serum ghrelin in untreated hyperthyroidism were significantly lower than those in hyperthyroid patients achieved aeuthyroid status (t=3.21,P<0.01). hypothyroidism (t=3.02,P<0.01) and the normal gruups (t=3.39,P<0.01),but the levels of serum ghrelin in hypothyroidism patients were similar to those in normal groups;(2) The levels of sernm leptin did not differ; (3) Serum ghrelin levels were negatively correlated with FT3 (r=-0.29,P<0.05) and,FT4 (r=-0.26,P<0.05),positively correlated with serum TSH (r=0.36,P<0.05);serum leptin levels did not correlate with thyroid hormone.Conclusion The levels of serum ghrelin Welm,differently under different thyroid funotional status and correlated with thyroid hormone,while serum leptin were not.
Objective To investigate the effect of different status of thyroid function (hypothyroidism and hyperthyroidism as well a8 euthyroid status)on serum ghrelin and leptin levels.Methods The levels of semm ghrelin and leptin were determined by radio immunoassay in 46 untreated subjects with hyperthyroidism.15 hyperthyroid patients achieved aeuthyroid status after radioiodine 131I therapy,21 cases of hypothyroidism and 18 cases of normal controls,respectively.Meanwhile,the serum levels of free triiodothyronine (FT3),free thyroxine (FT4) and thyroid-stimulating hormone (TSH) were measured by chemiluminescence immune assay.Results (1) The levels of serum ghrelin in untreated hyperthyroidism were significantly lower than those in hyperthyroid patients achieved aeuthyroid status (t=3.21,P<0.01). hypothyroidism (t=3.02,P<0.01) and the normal gruups (t=3.39,P<0.01),but the levels of serum ghrelin in hypothyroidism patients were similar to those in normal groups;(2) The levels of sernm leptin did not differ; (3) Serum ghrelin levels were negatively correlated with FT3 (r=-0.29,P<0.05) and,FT4 (r=-0.26,P<0.05),positively correlated with serum TSH (r=0.36,P<0.05);serum leptin levels did not correlate with thyroid hormone.Conclusion The levels of serum ghrelin Welm,differently under different thyroid funotional status and correlated with thyroid hormone,while serum leptin were not.
2008, 32(4): 224-226.
Abstract:
Objective To obtain the level of IL-1β in auto-immue pathological processes of thyroid eye disease patients treated with 131I.Methods By the prepositive dignose,a total of 31 patients of thyroid eye disease was investigated.They all had opthalmic symptoms,their thyroid hormones were higher than that of normal persons.These patients were foreclosed the ophthalmology caused by other diseases making use of the orbit CT.The degree of binocular exopthalmos was measured by a specialist.The dosage of 131I treatment according to formula calculation.To detect the serum level of IL-1β by rdioimmunoassay.Results The serum level of IL-1β in thyroid eye disease group is higher than that of control group in some degree (pretherapeutic level of IL-1β is (0.15±0.07)ng/ml,therapeutic level of IL-1β is (0.11±0.05) ng/ml,normal control is (0.10±0.03) ng/ml,H=68.088,P<0.001.After 131I treatment,serum level of IL-1β were dropped in thyroid eye disease patients (H=88.56,P<0.001),and the degree of ophthalmic symptom was also mended. Conclusion The level of IL-1β in peripheral blood of thyroid eye disease patients is closely associated with morbility,and there is a reduction of IL-1β level in the serum of thyroid eye disease patient after 131I treatment compared with that before treatment,also there is a significant improvement after treatment.
Objective To obtain the level of IL-1β in auto-immue pathological processes of thyroid eye disease patients treated with 131I.Methods By the prepositive dignose,a total of 31 patients of thyroid eye disease was investigated.They all had opthalmic symptoms,their thyroid hormones were higher than that of normal persons.These patients were foreclosed the ophthalmology caused by other diseases making use of the orbit CT.The degree of binocular exopthalmos was measured by a specialist.The dosage of 131I treatment according to formula calculation.To detect the serum level of IL-1β by rdioimmunoassay.Results The serum level of IL-1β in thyroid eye disease group is higher than that of control group in some degree (pretherapeutic level of IL-1β is (0.15±0.07)ng/ml,therapeutic level of IL-1β is (0.11±0.05) ng/ml,normal control is (0.10±0.03) ng/ml,H=68.088,P<0.001.After 131I treatment,serum level of IL-1β were dropped in thyroid eye disease patients (H=88.56,P<0.001),and the degree of ophthalmic symptom was also mended. Conclusion The level of IL-1β in peripheral blood of thyroid eye disease patients is closely associated with morbility,and there is a reduction of IL-1β level in the serum of thyroid eye disease patient after 131I treatment compared with that before treatment,also there is a significant improvement after treatment.
2008, 32(4): 227-229.
Abstract:
Objective To evaluate the effect of high concentration of thyroid hormone and cell growth factor content on the bone metabolism of hyperthyreosis.Methods Radiation immunological test and chemiluminescence methods are employed to determinate the content of free triiodothyronine (FT3),free thyroxine (FT4),thyroid stimulating hormone (TSH),insulin-like growth factor Ⅱ (IGF-Ⅱ),calcitonic (CT), interleukin-6 (IL-6) and tumor necrosis factor (TNF) of serum in health adult and parts of hyperthyreosis patients.Results Hyperthyreosis patients have a higher content of FT4,FT3 and IL-6 than those of health adult(t was 16.69,11.33,7.92,respectively,P<0.01),while the content of TSH,IGF-Ⅱ,CT,TNF are obvious deceasing (t was 13.08,8.34,5.29,8.75,respectively,P<0.01).Conclusion In patients with hyperthyreo-sis,high concentration thyroid hormone cause accentuation of protein metabolism,decrease calcium homeostasis by disorders of phosphorus and calcium metabolism,high concentration thyroid hormone and low level CT resulted in bone loss.Decreased IGF-Ⅱ may be the main cause of osteoporosis as the resuh of high concentration thyroid hormone.
Objective To evaluate the effect of high concentration of thyroid hormone and cell growth factor content on the bone metabolism of hyperthyreosis.Methods Radiation immunological test and chemiluminescence methods are employed to determinate the content of free triiodothyronine (FT3),free thyroxine (FT4),thyroid stimulating hormone (TSH),insulin-like growth factor Ⅱ (IGF-Ⅱ),calcitonic (CT), interleukin-6 (IL-6) and tumor necrosis factor (TNF) of serum in health adult and parts of hyperthyreosis patients.Results Hyperthyreosis patients have a higher content of FT4,FT3 and IL-6 than those of health adult(t was 16.69,11.33,7.92,respectively,P<0.01),while the content of TSH,IGF-Ⅱ,CT,TNF are obvious deceasing (t was 13.08,8.34,5.29,8.75,respectively,P<0.01).Conclusion In patients with hyperthyreo-sis,high concentration thyroid hormone cause accentuation of protein metabolism,decrease calcium homeostasis by disorders of phosphorus and calcium metabolism,high concentration thyroid hormone and low level CT resulted in bone loss.Decreased IGF-Ⅱ may be the main cause of osteoporosis as the resuh of high concentration thyroid hormone.
2008, 32(4): 230-231.
Abstract:
Objective To investigate the effect of 131I therapy Graves disease (GD) complicated with pefipheral blood leukopenia in hyperthyroid patients.Methods Serum T3,T4,TSH levels and peripheral blood white blood cell counts were determined in 16 GD complicated with leukopenia patients both before and after 131I therapy.Results 131I treatment of GD complicated with leukopenia patients was very successful (P<0.01),the cured rate was 100%.The peripheral blood white blood cell counts were few changes in GD patients both before and after 131I T3,T4,TSH levels was decreased within three months after 131I therapy.Conclusion The application of 131I to treat GD complicated with leukopenia was very successful.The therapeutic dose of 131I was no remarkable effect on peripheral blood white blood cell.
Objective To investigate the effect of 131I therapy Graves disease (GD) complicated with pefipheral blood leukopenia in hyperthyroid patients.Methods Serum T3,T4,TSH levels and peripheral blood white blood cell counts were determined in 16 GD complicated with leukopenia patients both before and after 131I therapy.Results 131I treatment of GD complicated with leukopenia patients was very successful (P<0.01),the cured rate was 100%.The peripheral blood white blood cell counts were few changes in GD patients both before and after 131I T3,T4,TSH levels was decreased within three months after 131I therapy.Conclusion The application of 131I to treat GD complicated with leukopenia was very successful.The therapeutic dose of 131I was no remarkable effect on peripheral blood white blood cell.
2008, 32(4): 232-235.
Abstract:
Objective To explore the dynamic of high-sensitivity C-reactive protein (hs-CRP) and its correlation with insulin resistance (IR) during different stages of gestational diabetes mellitus (GDM). Methods Thirty-two subjects with GDM and thirty-one cases of normal glucose tolerance (NGT) pregnant women were enrolled in the study,fasting insulin (FINS) were determined by radioimmunoassay,the fasting blood glucose (FBG) was measured by using glucose oxidase,immunoturbidimetry performed to evaluate serum hs-CRP levels.Tests repeated for each group according different stages of prenatal 25-28 weeks,29-32 weeks,37-38 weeks and postpartum 6-8 weeks.IR was assessed by the homeostasis model assessment insulin resistance index (HOMA-IR),Results ①Serum hs-CRP levels in NGT and GDM had few change with gestational age,but both significantly decreased at postpartum.②Serum hs-CRP levels in GDM significantly increased than NGT in the same stage (t was 7.31,7.78,6.33,respectively,P<0.01).③HOMA-IR in GDM significantly increased than NGT in the same gestational stage (t was 31.18,31.10, 28.39,respectively,P<0.01).④Pearson correlation analysis showed that FBG,FINS and hs-CRP had significant association with HOMA-IR (regression coefficient of 0.478,0.902,0.293,respectively,P<0.01). Multiple regression analysis identified FINS,FBG and hs-CRP as the factors significantly affecting HOMA-IR (regression coefficient of 0.441,0.876,0.261,respectively,P<0.01).Conclusion Serum hs-CRP levels in GDM had few change with gestational age,but all significantly increased than NGT in the same stage,and which were the most significant factors affecting HOMA-IR.
Objective To explore the dynamic of high-sensitivity C-reactive protein (hs-CRP) and its correlation with insulin resistance (IR) during different stages of gestational diabetes mellitus (GDM). Methods Thirty-two subjects with GDM and thirty-one cases of normal glucose tolerance (NGT) pregnant women were enrolled in the study,fasting insulin (FINS) were determined by radioimmunoassay,the fasting blood glucose (FBG) was measured by using glucose oxidase,immunoturbidimetry performed to evaluate serum hs-CRP levels.Tests repeated for each group according different stages of prenatal 25-28 weeks,29-32 weeks,37-38 weeks and postpartum 6-8 weeks.IR was assessed by the homeostasis model assessment insulin resistance index (HOMA-IR),Results ①Serum hs-CRP levels in NGT and GDM had few change with gestational age,but both significantly decreased at postpartum.②Serum hs-CRP levels in GDM significantly increased than NGT in the same stage (t was 7.31,7.78,6.33,respectively,P<0.01).③HOMA-IR in GDM significantly increased than NGT in the same gestational stage (t was 31.18,31.10, 28.39,respectively,P<0.01).④Pearson correlation analysis showed that FBG,FINS and hs-CRP had significant association with HOMA-IR (regression coefficient of 0.478,0.902,0.293,respectively,P<0.01). Multiple regression analysis identified FINS,FBG and hs-CRP as the factors significantly affecting HOMA-IR (regression coefficient of 0.441,0.876,0.261,respectively,P<0.01).Conclusion Serum hs-CRP levels in GDM had few change with gestational age,but all significantly increased than NGT in the same stage,and which were the most significant factors affecting HOMA-IR.
2008, 32(4): 236-239.
Abstract:
Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow eytometry.Results The PDT of SHG-44 cells before irradiation Was (22.78±2.61)h.The PDT of irradiated SHG-44 ceils at dose of 10 Gy were (30.46±2.73)h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant G2/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.
Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow eytometry.Results The PDT of SHG-44 cells before irradiation Was (22.78±2.61)h.The PDT of irradiated SHG-44 ceils at dose of 10 Gy were (30.46±2.73)h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant G2/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.
2008, 32(4): 240-243.
Abstract:
Platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) are abnormally synthesized following the autocrine growth activation in a number of malignant tumors.PDGF is a effective mitogen and chemical agent,PDGFR belongs to tyrosine kinase receptors.After binding with corresponding ligand,PDGFR is activated and lead to activate a series of downstream signal pathways and has resulted in many biological effects.PDGF and PDGFR signal pathway is associated with radiation resistance of tumor cells,and the mechanism is likely to be related to cell proliferation,suppressing apoptosis,and regulating cell cycle arrest.
Platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) are abnormally synthesized following the autocrine growth activation in a number of malignant tumors.PDGF is a effective mitogen and chemical agent,PDGFR belongs to tyrosine kinase receptors.After binding with corresponding ligand,PDGFR is activated and lead to activate a series of downstream signal pathways and has resulted in many biological effects.PDGF and PDGFR signal pathway is associated with radiation resistance of tumor cells,and the mechanism is likely to be related to cell proliferation,suppressing apoptosis,and regulating cell cycle arrest.
2008, 32(4): 243-246.
Abstract:
The biological effects of ionizing irradiation is related with dose and dose-rate.Medium to large dose irradiation can induce damaging effect to biosystem.but low dose radiation may induce adaptive response,hormesis,hyper.sensitiVeness and bystander effeeL Especially experimental data suggest that the antitumor effects of immune enhancement,intrinsic hypersensitivity,activation antioxidase enzymatic system and induction of apoptosis is produced by low-dose total body irradiation.Furthermore it call decrease relative cancer risk in exposed populations.Therefore.the antitumor effect by low-dose irradiation is being widely investigated and intensively researched.
The biological effects of ionizing irradiation is related with dose and dose-rate.Medium to large dose irradiation can induce damaging effect to biosystem.but low dose radiation may induce adaptive response,hormesis,hyper.sensitiVeness and bystander effeeL Especially experimental data suggest that the antitumor effects of immune enhancement,intrinsic hypersensitivity,activation antioxidase enzymatic system and induction of apoptosis is produced by low-dose total body irradiation.Furthermore it call decrease relative cancer risk in exposed populations.Therefore.the antitumor effect by low-dose irradiation is being widely investigated and intensively researched.
2008, 32(4): 247-250.
Abstract:
Tumor treatment is one of the most important fields in medical research.Nowadays, a novel method which is combined gene therapy with radiotherapy plays an important role in the field of cancer research,and mainly includes immune gene therapy combined with radiotherapy,suicide gene therapy or tumor suppressor gene therapy combined with radiotherapy,antiangiogenesis gene therapy combined with radiotherapy and protective gene therapy combined with radiotherapy based on the technical features.This review summarized the current status of combined therapies of gene therapy and radiotherapy and possible mechanism.
Tumor treatment is one of the most important fields in medical research.Nowadays, a novel method which is combined gene therapy with radiotherapy plays an important role in the field of cancer research,and mainly includes immune gene therapy combined with radiotherapy,suicide gene therapy or tumor suppressor gene therapy combined with radiotherapy,antiangiogenesis gene therapy combined with radiotherapy and protective gene therapy combined with radiotherapy based on the technical features.This review summarized the current status of combined therapies of gene therapy and radiotherapy and possible mechanism.
2008, 32(4): 250-254.
Abstract:
Radiation-induced brain injury is awell-described complication of brain and neck cancers radiotherapy.With the increasing use of radiotherapy for brain and neck cancers in recent years,the incidence of radiation-induced brain injury which has influenced the sufferer's living quality and existence period seriously has markedly increased.As a result,the research to the radiation-induced brain injury seems to be more urgently.The article reviewed the mechanism and treatment and wanted to provide areference for the treatment which reduces incidence of radiation-induced brain injury.
Radiation-induced brain injury is awell-described complication of brain and neck cancers radiotherapy.With the increasing use of radiotherapy for brain and neck cancers in recent years,the incidence of radiation-induced brain injury which has influenced the sufferer's living quality and existence period seriously has markedly increased.As a result,the research to the radiation-induced brain injury seems to be more urgently.The article reviewed the mechanism and treatment and wanted to provide areference for the treatment which reduces incidence of radiation-induced brain injury.
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