Radiosensitivity of filial generation from irradiated human glioma cell line SHG-44

Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow eytometry.Results The PDT of SHG-44 cells before irradiation Was (22.78±2.61)h.The PDT of irradiated SHG-44 ceils at dose of 10 Gy were (30.46±2.73)h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant G₂/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.