冰冻切片放射自显影技术在微剂量研究中的应用

Application of study in microdosimetry with the frozen sectioning microaut-oradiogtraphy technique

摘要: 目的用放射自显影技术观察放射性核素在细胞水平的分布。方法采用放射自显影和冰冻切片技术,建立银颗粒密度与放射性药物强度的刻度曲线,确定放射性药物¹³¹I和^99mTc的微观分布,基于核素的微观分布数据,建立微剂量的剂量估算模式。结果银颗粒密度与施入放射性药物比活度的相关系数分别为0.9915和0.9963,刻度系数分别为1.59×10^-4Bq和6.48×10^-5Bq。结论 ¹³¹I和^99mTc在细胞水平的分布是不均匀的,银颗粒多数分布在细胞质中,因此在计算细胞水平的剂量时应考虑到其分布的不均匀性。

Abstract: Objective To survey the distribution of radionuclide in cell level by frozen sectioning microautoradiogtraphy technique. Methods The article will describe the micro distribution of ¹³¹I and ^99mTc using autoradiogtraphy and frozen sectioning technique. Results To describe the relationship between argent grain density and nuclide radioactivity a regression curve of ^99mTc and ¹³¹I was depicted and the R value calculated to be 0.9915 and 0.9963 respectively, The scale coeflqcient of argent grain were 1,59×10^-4Bq and 6.48×10^-5Bq respectively. Conclusion The distribution of radiomedicament ¹³¹I and ^99mTc at cellular level is nonunidoem.