Abstract: Prostate cancer is one of the most common malignancies in old men, positron emission tomography is very valuable in early diagnosing and accurately staging of prostate cancer. ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG)PET is not sensitive in detection and reliable on staging of prostate cancer, but the uptake of ¹⁸F-FDG correlates with the stage of prostate cancer. ¹¹C-choline PET is accurate in diagnosis of prostate cancer, and it is superior to ¹⁸F-FDG PET in detection of metastases in lymph nodes and bone. But its short half-life limits the clinic applying in prostate cancer. The uptake of ¹⁸F-choline in prostate cancer is similar to ¹¹C-choline, but its excretion in urinary system is much higher than ¹¹C-choline. The other tracers such as ¹¹C-acetate, ¹¹C-methionine and 16β-¹⁸F-fluoro-5α-dihydrotestosterone are also valuable in diagnosing, staging and evaluating the effect of treatment of prostate cancer.