Abstract: Myocardial perfusion SPECT is a relative accurate uninvasive method of detecting coronary heart disease and ischemia myocardiopathy,which is traditionally using ⁹⁹Tc^m-MIBI、²⁰¹Tl as well as ¹⁸F-FDG.Futher,in order to resolve the disadvantages of mono-isotope myocardial perfusion SPECT,more and more attention are payed to radiopharmaceuticals、method and application of dual-isotope myocardial perfusion SPECT and great achievement has been gotten.