Abstract: Objective To discuss the influence of radioactive seeds implantation and radiotherapy on VX₂ tumor cytokine. Methods 60 male New Zealand White rabbits were randomly divided into 3 groups, 20 for radioactive seeds implantation group, 20 for radiotherapy group, and 20 for control group. After 12d the implantation with the VX soft tumor, radioactive seeds implantation and radiotherapy were performed separalely, the control group underwent no therapy. IL-2, IL-6, IL-8, TNF and IGF were measured. Results The survival periods of experimental group were 15d longer than the control group. The concentration of IL-2, IL-8 in serum decreased gradually, the decreasing extent control > radiotherapy > radioactive seeds implantation;the concentration of IL-6, IGF raised gradually, rising extent control > radiotherapy > radioactive seeds implantation. Conclusions The immune system of rabbits with VX₂ tumor were suppressed, there is little influence of radioactive seeds implantation and radiotherapy on cytokine. Radioactive seeds implantation and radiotherapy are effective methods to treat VX₂ tumor.