Abstract: Objective To study on the gene expression of normal lymphoblastoid cells(AHH-l) which exposure to difference dose of ⁶⁰Coγ-ray, analyses the essential different biological effect. Methods Human AHH-I normal line was irradiated by ⁶⁰Coγ-rays. Used human cDNA microarray to develop the transcriptional levels of the genes by hybridizing the mRNA of cells 8 h after exposured in different dose and the control cells. Cluster analysis, discrimination and bolting were used to filter the effective genes of differential expression. Results The results of data analysis showed 23 genes of differential expression closely related to biological effect of 2.0 Gy radiation, 5 genes express changed only by 0.5 Gy radiation, 5 genes express apparently both in 2.0 Gy and 0.5 Gy radiation. Conclusion The different dose γ-rays radiation-induced significant changes in gene expression, such as PAPLN, TP53INPI, PTENP1, FOS and TPR seem to be some important components of cellular radioresponse.