Abstract: Radionuclide bone imaging is used as the diagnostic procedure of choice for diagnosis of bone and soft-tissue infection and can aid in the diagnosis of occult trauma without radiographic findings. There is a complimentary role in the assessment of a child with suspected non accidental injury. It also may provide a diagnosis that could be related to trauma, tumor, or inflammation in a child with unexplained bone pain or limp. Radionuclide bone imaging in children require careful attention to technique to obtain high quality diagnostic images. Routine whole-body imaging, magnification, additional views, and the use of single-photon emission computed tomography also are a routine part of this examination in children. Correlation with conventional radiographs is mandatory, and the judicious use of hybrid imaging with the addition of computed tomography may further improve diagnostic acumen, confidence and accuracy. New radiopharmaceuticals such as Na¹⁸F may also play a role in changing techniques for pediatric radionuclide bone imaging.