Abstract: Radionuclide antisense therapy achieves the joint goals of antisense therapy and internal radioation therapy.There have been a small number of investigations on the radionuclide antisense therapy in tissue culture and in animal studies.Considerable research is required before this novel technique can become a working practice.We reviewed some question and development on the radionuclide antisense therapy,such as selection of the target gene sequence,labelling the antisense oligonucleotide,improvement of the uptake and target of radionuclide antisense oligonucleotides and evaluation of the toxicity of the radionuclide antisense therapy.