Abstract: Objective To explore the changes and clinical significance Of TRAb and TSI detection in patients with Graves' disease. Methods Serum TRAb and TSI levels were detected by enzyme-linked immunesorbent assay, and thyroid hormone levels were detected by micropaticle enzyme immuneoassay, including normal controls, Graves' disease in period of onset catabsis group and hashimotos thyroiditis group. Results The positive rate of TRAb and TSI in Graves' in period of onset group is 86.67% and 95.0%, TGA and TMA in hashimotes thyroiditis group is 85.29% and 91.18%, respectively. More importantly these results were significant difference than normol controls. Conclusions It's very important for Graves' disease patients to detect TRAb, TSI, TGA, TMA and thyroid hormone simulta-neously, especially to the curative effect and prognosis criterin in patients with Graves' disease and antidi-astole in patients with hashimotos thyroiditis.