Abstract: Radionuclide imaging of infection and inflammation is a powerful diagnostic tool in the management of patients with infectious or inflammatory disease. Because of the excellent performance, radiolabeled leukocytes have become a gold standard nuclear medicine technique to image infection and inflammation. However, the preparation of this radiopharmaceutical is laborious and requires the handling of potentially contaminated blood. A few other methods are available that could be used instead of radiolabeled leukocytes imaging. In this article, the characteristics and diagnostic potential of established and experimental scintigraphic methods of infection and inflammation are reviewed.