Abstract: Magnetic resonance spectroscopy as a technology for measuring response of living systems to pathophysiological stimuli is conducted on biofluids (e.g. urine or blood), cell supernatants or tissue samples and can be regarded as an effective and non-invasively metabolic analysis method. In this review, it is shown how the technique of proton magnetic resonance spectroscopy can be applied to physiological evaluation, drug safety assessment and disease diagnosis and its assessment of the possible future role is also presented.