Abstract: Single cell gel electrophoresis(SCGE), which has founded in 1980s, can detect the DNA injury in single cell. Studies on radiation biology has indicated that we can detect the DNA injury by SCGE in mammal cells such as sperm cell, tumor cell and lymphocyte after radiation. Recently, along with the rapid progress of technology, new software has been exploited, which adds much new meanings to this method. SCGE reveals a great future in study of radiation biological dosimetry and has more ad-vantage than the other traditional methods, particularly at the early stage after radiation.