Abstract: Objective In this study,we explored the potential clinical application of the instrument for automatic distribution of isotopes in order to reduce the injuries to operators by radiation.Methods The accuracy of samples and activities of isotopes were determined by acquisition of the samples through the instrument distributing multiple samples at different volumes.In addition,the therapeutical effects on the patients with thyroidism were compared between those who were treated by the isotope distributed via the instrument(IDI group)and those who were treated by the isotope distributed manually(IDM group).Results Acquisition errors by the instrument were under 0.1ml and the isotope distribution didn't affect the activity of radiation.Moreover,no significant difference was observed between the IDI group and the IDM group.Conclusion Isotope distribution by the instrument is accurate and reliable.And by this proceeding,radiation injuries to operators are decreased.