Abstract: Patient suffered from differentiated thyroid carcinoma(DTC)needs lifetime follow-up even after going through the thyroidectomy,postoperative ¹³¹I treatment,thyroid stimulating hormone suppressing therapy.There are disadvantages in DTC routine follow-up,such as conventional radiologieal studies,measurement of serum thyrogiobulin and ¹³¹I scintigraphy.It is not rare for us to see patients with dedifferentiated metastases which with no radioiodine uptake,and how to locate these dedifferentiated metastases is of great importance for making further treatment plan.Tumor imaging agent ⁹⁹Tc^m-methoxyisobutylisonitrile(⁹⁹Tc^m-MIBI)is widely used in the clinical and laboratories research of DTC in domestic and overseas,for its excellent physical and dosimetric characteristics and reasonable price.This review relates the application of ⁹⁹Tc^m-MIBI imaging in DTC follow-up,and focuses on its imaging mechanism,clinical methods,imaging interpretation,and the advantages.