Abstract: At present, there are many methods for detecting apoptosis in vitro, but these methods just are traumatic in material extraction and tissue biopsy by sacrificing of animals in vitro, which limits their clinical application and transformation. In vivo detection methods have become the focus of current research because of non-invasive and real-time monitoring of apoptosis in vivo. Radionuclide apoptotic cell imaging technology among has good research prospects in non-invasive, early stage, dynamic, sensitive, quantitative, and detection intravital. It is the most widely studied and mature technology for detecting apoptosis intravital. Radionuclide apoptosis imaging has been widely used in the detection of apoptosis in cardiovascular diseases, central nervous system diseases, organ transplant rejection, and the evaluation of efficacy and prognosis of malignant tumor after radiotherapy and chemotherapy. This article reviews the progress of radionuclide imaging probes in apoptosis.