Abstract: Glioma is one of the common diseases of the nervous system, and it poses hazards to human health.Prognosis of glioma depends on staging, surgical resection, histological classification, and pathological grading.Both ¹¹C-L-methionine and ¹⁸F-fluoroethyl tyrosine are popular amino acid tracers used in PET imaging of brain tumors and reflect amino acid transport, metabolism, and protein synthesis in vivo.A literature overview on the role of amino acid PET in glioma is provided in terms of diagnostic accuracy, grading, differentiation of tumor recurrence and radiation necrosis, radiotherapy planning, and prognosis.