Abstract: Radiation-induced intestinal injuries, a devastating adverse effect of radiation therapy, can lead to local ischemia and fibrosis with the development of ulcers, strictures and lower gastrointestinal bleeding. As the complex causes of radiation-induced intestinal injuries, diverse evaluating methods and animal model had been established. In this context, the present review will focus on the evaluating and establishing methods of radiation-induced intestinal injuries animal model, what would provide ideas for related basic research.