免疫诱导IRM-2小鼠再生障碍性贫血模型的研究

Immune-induced aplastic anemia IRM-2 mice model research

    摘要
  • 摘要:
    目的 建立IRM-2小鼠再生障碍性贫血(AA)实验动物模型, 并检测其血液及相关免疫学指标。
    方法 IRM-2小鼠经137Cs γ射线6 Gy照射后, 输入DBA/2小鼠的胸腺细胞, 建成免疫介导型AA模型。实验分3组, 即对照组、照射组和AA模型组, 于照射后第15天检测小鼠的血常规和免疫学指标。
    结果 IRM-2小鼠AA模型组外周血WBC、RBC、骨髓有核细胞数和网织红细胞百分比分别为(2.38±0.53)×109/L、(8.22±0.21)×1012/L、(7.14±1.19)×106/股骨和(50.2±8.9)‰, 均低于对照组(8.23±0.41)×109/L、(10.24±0.91)×1012/L、(16.5±1.61)×106/股骨和(76.2±9.7)‰; IRM-2小鼠AA模型组免疫学指标中CD4+细胞比例(8.91±2.55)%低于对照组(16.14±3.09)%, CD8+细胞比例(13.55±2.12)%高于对照组(6.83±1.14)%。IRM-2小鼠AA模型组外周血及免疫学指标与对照组比较, 差异均具有统计学意义。
    结论 IRM-2小鼠AA动物模型的建立, 对AA的研究具有较好的应用价值。

     

    Abstract:
    Objective To perform traditional blood and immunologic examinations for the pathogenesis of immune-induced aplastic anemia(AA) IRM-2 mice model.
    Methods IRM-2 mice were divided into three groups, namely, the control, radiation, and AA model groups. IRM-2 inbred mice in the AA model group were used to set up the AA model by whole body 137Cs γ irradiation and eye venous plexus injection with DBA/2 mice thymus cells. After 15 days of radiation, blood and bone marrow cells were counted, and immunologic detection was performed.
    Results The peripheral WBC, RBC, bone marrow cells, and reticulocyte(2.38±0.53)×109/L, (8.22±0.21)×1012/L, (7.14±1.19)×106/femur, and(50.2±8.9)‰ in the AA model group were lower than those of the control group (8.23±0.41)×109/L, (10.24±0.91)×1012/L, (16.5±1.61)×106/femur, and(76.2±9.7)‰. CD4+ cells (8.91±2.55)% of the AA model group were lower than those of the control group(16.14±3.09)%, whereas CD8+ cells (13.55±2.12)% were higher than those of the control group (6.83±1.14)%. Peripheral blood and immunological indexes of the AA model group were statistically different from those of the control group.
    Conclusion IRM-2 mice AA model was successfully established as a good model for the study of AA.

     

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