siRNA干扰SIRT1对辐射后IL-6表达的影响

Effect of SIRT1 siRNA interference on the expression of IL-6 after radiation

    摘要
  • 摘要:
    目的 研究siRNA干扰沉默信息调节因子2相关酶1（SIRT1）对间充质干细胞（MSCs）电离辐射后诱导的炎性因子白细胞介素6（IL-6）的影响，探究SIRT1的辐射防护作用。
    方法 使用0、2、4、8 Gy照射MSCs，分别在照后3、6、12、24 h提取总RNA和总蛋白，检测IL-6表达水平；将MSCs分为空白对照组、单纯照射组和SIRT1干扰联合照射组，使用Western blot、RT-PCR检测SIRT1和IL-6胞内表达。
    结果 MSCs在电离照射后，IL-6的表达水平先升高后降低；在照后12 h达到最高，24 h恢复基准水平；而联合SIRT1干扰会引起IL-6水平进一步升高，加重MSCs电离辐射后的炎症反应。
    结论 SIRT1可能通过抑制电离辐射诱导的炎性因子IL-6的表达，发挥辐射防护作用。

     

    Abstract:
    Objective To investigate the effect of the knockdown of ilent information regulation factor 2 related enzyme on inflammatory factor IL-6 produced in irradiated mesenchymal stem cells(MSCs) and explore the radioprotective effect of SIRT1.
    Methods MSCs was treated with different dose of irradiation, and the expression of IL-6 in different time was detected by Western-blot and RT-PCR; MSCs were divided into control group, radiation group and combination group, the expression of IL-6 and SIRT1 was detected by Western-blot and RT-PCR.
    Results Ionizing radiation could lead to the increasing of IL-6 expression in MSCs. In detail, the expression level of IL-6 protein reached the peak in 12 hours after radiation and then slowly recovered to the baseline in 24 hours after radiation. And interfered with SIRT1 could aggravate the expression of ionizing radiation-induced IL-6 and worsen inflammation.
    Conclusion SIRT1 may inhibit the ionizing radiation-induced expression of inflammatory cytokines IL-6.

     

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