姜油树脂对辐射后间充质干细胞Nrf2及其靶基因表达的影响

Ginger oleoresin induced expression of Nrf2 and its target genes in mesenchymal stem cells after radiation

    摘要
  • 摘要:
    目的 探索姜油树脂对辐射后间充质干细胞中核转录因子E2相关因子2（Nrf2）及其下游靶基因表达变化的影响。
    方法 采用MTT、实时PCR以及Western Blot检测不同浓度姜油树脂、不同时间点处理后Nrf2及其下游靶基因血红素加氧酶（HO1）和还原型辅酶Ⅰ醌类氧化还原酶（NQO1）的表达变化。
    结果 姜油树脂对辐射后间充质干细胞中Nrf2基因本身的影响并不大，但在给予姜油树脂24 h后，10-5和10-4 g/ml姜油树脂联合照射组下游靶基因HO1和NQO1的mRNA及蛋白水平均有所上升。
    结论 姜油树脂不影响Nrf2的转录和表达，但可提高其下游抗氧化基因的表达，发挥辐射防护作用。

     

    Abstract:
    Objective To explore ginger oleoresin induced the change of expression of nuclear factor-erythroid 2 p45-related factor 2(Nrf2) and its target genes in mesenchymal stem cells after radiation damage.
    Methods A combined approach include MTT, real time-PCR and Western blot were used to test the changes on expression level of Nrf2 and its downstream genes, including heme oxygenase-1(HO1) and NAD(P)H: quinone oxidoreductase 1(NQO1).
    Results The results showed that ginger oleoresin has little effect on Nrf2. However, the expression of HO1 and NQO1 increased in the 10-5, 10-4 g/ml ginger oleoresin group when incubated 24 h after radiation.
    Conclusions Ginger oleoresin has no effect on the transcription and expression of Nrf2, but can increase the expression of the target antioxidant genes of Nrf2. Ginger oleoresin may be a potential protective agent in the future.

     

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