12C重离子与γ射线对人外周血淋巴细胞Peng-EBV增殖、细胞周期及凋亡影响的比较

Effects of 12C heavy ion and γ ray irradiation in cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes

    摘要
  • 摘要:
    目的 比较12C重离子与γ射线对人外周血淋巴细胞Peng-EBV增殖抑制率、细胞周期和凋亡率的影响。
    方法 对Peng-EBV细胞分别进行2.0 Gy的12C重离子和γ射线照射，通过MTS法检测细胞增殖变化，流式细胞术检测细胞凋亡率和周期分布。
    结果 2.0 Gy重离子射线和γ射线作用后，Peng-EBV细胞的增殖抑制率均显著升高。与对照组相比，2.0 Gy射线照射后24、48、72 h细胞增殖抑制率差异有统计学意义（重离子射线：t=10.53、4.11、37.30，P＜0.05；γ射线：t=7.18、8.26、26.11，P＜0.05）。12C重离子对细胞的增殖抑制率在照射后72 h内呈时间依赖性升高，而γ射线则在照射后48 h时增殖抑制率最高，而后降低。重离子射线照射后12 h和24 h，细胞凋亡率分别较对照组增加了7.92和13.41倍（t=7.09、4.13，P＜0.05）；而γ射线照射后12 h和24 h，细胞凋亡率分别为对照组的2.43和5.64倍（t=8.98、20.52，P＜0.05）。重离子射线照射后12 h和24 h，细胞G2/M期阻滞程度分别较对照组增加5.11和11.77倍（t=12.06、100.08，P＜0.05）；而γ射线照射后12 h和24 h，细胞G2/M期阻滞程度分别为对照组的5.82和7.68倍（t=19.35、10.30，P＜0.05）。
    结论 12C重离子对人外周血淋巴细胞造成的生物学效应远高于γ射线。

     

    Abstract:
    Objective To compare the effects of carbon heavy ion and γ ray on cell survival, cell cycle and cell apoptosis.
    Methods Peng-EBV cells were irradiated by carbon heavy ion and X-ray. MTS was used to detect the cell survival. Cell cycle and apoptosis were analyzed by flow cytometry.
    Results The proliferation inhibition rate of Peng-EBV cell was significantly increased after 2.0 Gy heavy ion rays and γ rays. Compared with the control group, the cell proliferation inhibition rate difference was statistically significant after 2.0 Gy rays at 24 h, 48 h, 72 h(heavy rays:t=10.53, 4.11, 37.30, all P < 0.05; γ rays:t=7.18, 8.26, 26.11, all P < 0.05). The cell proliferation inhibition rate showed a time-dependent increase, and reached its peak at 72 h after heavy ion rays, while the cell proliferation inhibition rate after γ ray reached its peak at 48 h, and then decreased. Compared with the control group, the apoptosis rate of Peng-EBV cell increased 7.92 and 13.41 times respectively at 12 h and 24 h after 2.0 Gy heavy ion rays(t=7.09, 4.13, both P < 0.05), while it was 2.43 and 5.64 times after 2.0 Gy γ rays(t=8.98, 20.52, P < 0.05). Compared with the control group, the G2/M phase arrest increased 5.11 and 11.77 times respectively at 12 h and 24 h after 2.0 Gy heavy ion rays(t=12.06, 100.08, both P < 0.05), however, it was 5.82 and 7.68 times after 2.0 Gy γ rays(t=19.35, 10.30, both P < 0.05).
    Conclusion 12C heavy ion irradiation shows more stronger biological effects on human peripheral blood lymphocyte than γ ray.

     

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