电离辐射诱导小鼠骨电子顺磁共振信号检测的影响因素

任越; 张慧芳; 胡波; 王婧洁; 王宏利; 原雅艺; 张睿凤; 李幼忱

doi:10.3760/cma.j.issn.1673-4114.2015.05.004

电离辐射诱导小鼠骨电子顺磁共振信号检测的影响因素

Influencing factors of detection on ionizing radiation-induced electron paramagnetic resonance signal in bones

摘要

摘要:
目的研究影响小鼠骨辐射诱导电子顺磁共振（EPR）信号检测的因素。
方法将小鼠股骨去除软组织和骨髓，干燥，制成股骨段，经⁶⁰Co γ射线照射，照射剂量率为5 Gy/min，照射剂量为10 Gy和50 Gy。检测不同干燥程度、检测方向、微波功率和叠加次数等条件下骨EPR的信号强度。
结果降低骨样品含水量、设置合适的微波功率、增加叠加次数均可以增加样品信号强度，且不同检测方向下同一份样品的信号强度有一定差异。
结论适当的样品预处理和检测参数设定可以提高骨EPR检测的灵敏度，样品多方向检测并取平均值可减弱骨样品不均一产生的影响。

Abstract:
Objective To study the influencing factors of detection on ionizing radiation-induced electron paramagnetic resonance(EPR) signal in mice bone.
Methods Soft tissue and bone marrow samples of mice femur were collected, and femur sections were dried. The femur sections were irradiated at 10 and 50 Gy by ⁶⁰Co sources with irradiation dose rate of 5 Gy/min. Bone EPR signal intensity was detected under different degrees of drying, detection direction, and microwave power, as well as various scan times.
Results The water content of the bone sample was reduced, and microwave power was set to an appropriate value. Results showed that increasing scan times increased the signal intensity. Additionally, different detection directions of the same sample resulted in differences in signal intensity.
Conclusion Appropriate sample preparation and detection parameters can improve bone EPR detection sensitivity. Multidirectional detection of samples and averaging of the results may reduce the effects of inhomogeneity on bone samples.

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