Abstract: Objective To explore the diagnostic value of transrectal ultrasound-guided "6+X" points biopsy in prostate cancer. Methods All eligible databases were searched using the keywords, and the literature was evaluated by QUADAS. Statistical analysis was performed using Meta-Disc 1.4. Results Five studies satisfied the inclusion criteria. The transrectal ultrasound-guided "6+X" points biopsy in the diagnosis of prostate cancer exhibited the following characteristics: sensitivity, 0.84(95%CI, 0.79-0.89); specificity, 0.98(95%CI, 0.94-0.99); positive likelihood ratio, 26.06(95%CI, 2.87-236.68); negative likelihood ratio, 0.21(95%CI, 0.16-0.28); and diagnostic odds ratio, 170.95(95%CI, 55.68-524.84). The summary receiver operating characteristic curve indicated that the maximum joint sensitivity and specificity(Q^*-value) were 0.9625, and the Q index value was 0.9082. Conclusion Transrectal ultrasonography combined with "6+X" method had high clinical value in the early diagnosis of prostate cancer.