高效液相色谱与放射性流动计数器联用确证13N-氨水及测定放射化学纯度的研究

Identification of 13N-NH3 and analysis of its radiochemical purity using high performance liquid chromatography-ultraviolet-flow-count

    摘要
  • 摘要:
    目的 建立新的快速鉴别正电子显像剂13N-氨水及测定其放射化学纯度的有效方法。
    方法 采用高效液相色谱-紫外检测器联用放射性流动计数器（HPLC-UV-Flow-Count）的方法，以12.5%的氨水和0.1 mg/ml硝酸钠（NaNO3）作为标准液，AQ-C18柱为分离柱，流动相为5 mmol/L的辛烷磺酸钠水溶液和乙腈（体积比为3:1），流速为0.5 ml/min，紫外吸收波长为210 nm。将氨水和硝酸钠标准液进样检测，得到它们的色谱图后对比保留时间进行鉴别。
    结果 在上述的洗脱条件下，氨水和硝酸钠的UV保留时间分别为2.3和1.4 min，13N-氨水和杂质13N-NOx-的放射性保留时间分别为5.8和3.1 min，两种物质分离良好。放射性显像剂13N-氨水的放射化学纯度大于95%，符合临床应用的要求。
    结论 该方法简单、快速，适合半衰期极短的13N-氨水正电子显像剂的鉴别和放射化学纯度的测定。

     

    Abstract:
    Objective To establish a new, rapid, and effective method to identify 13N-NH3 and analyze its radiochemical purity.
    Methods The chromatographic method of high performance liquid chromatography-ultraviolet(HPLC-UV)-flow-count was performed using a C18 column with mobile phase of acetonitrile and 5 mmol/L sodium 1-octanesulfonate solution(V/V=1/3)at a flow rate of 0.5 ml/min and a wavelength of 210 nm. 15% 13N-NH3 and 0.1 mg/ml NaNO3 were detected separately, and their retention times were compared and identified.
    Results The UV retention times of NH3 and NaNO3 were 2.3 and 1.4 min, respectively, and the retention times of 13N-NH3 and 13N-NOx- were 5.8 and 3.1 min, respectively. The established separation method using HPLC-UV-flow-count showed good efficiency.
    Conclusion HPLC-UV-flow-count is a simple, valid, and rapid method to identify 13N-NH3 and analyze its radiochemical purity.

     

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