Abstract: As the cell therapy moves forward, adopting effective cell tracking techniques in living cells plays an important role in evaluating curative effects after cell therapy and monitoring differentiation and proliferation, migration and survival status of transplanted cells, which hold promise and potential to address many unmet clinical needs. So cell tracking technique in vivo is one of crucial techniques to transform cell therapy from basic research to clinical promoted application. This article is mainly to overview recent development and feasibility of cell tracking with optical and MR imaging.