G-CSF联合SB203580对4 Gy照射小鼠免疫系统的作用

李德冠; 路璐; 吴红英; 张俊伶; 孟爱民

doi:10.3760/cma.j.issn.1673-4114.2014.04.002

G-CSF联合SB203580对4 Gy照射小鼠免疫系统的作用

The effects of G-CSF combined with SB203580 on the immune system of mice received 4 Gy total body irradiation

摘要

摘要:
目的研究G-CSF联合p38抑制剂SB203580（SB）对免疫细胞辐射损伤的作用。
方法 C57BL/6雄性小鼠按体质量随机分为对照组、照射组和给药组。照射组和给药组给予4 Gy全身照射。给药组小鼠给予腹腔注射G-CSF和SB，G-CSF于4 Gy照射后2 h和6 h给药（1 μg/只），每天2次，连续给药5 d，SB于照射后24 h给药（15 mg/kg），隔日给药，共给药5次。照射后10 d测外周血计数，进行白细胞CD4、CD8、B220检测、胸腺细胞CD4、CD8检测和骨髓细胞活性氧检测。
结果照射组外周血计数和CD8、B220比例下降，而胸腺细胞中CD4⁺CD8⁺细胞比例及骨髓细胞活性氧水平显著增加。与照射组相比，联合给药组外周血红细胞数、血小板、CD4、CD8细胞比例升高，胸腺细胞中CD4⁺CD8⁺细胞比例下降。
结论 G-CSF联合SB对4 Gy照射引起的免疫细胞损伤存在保护作用。

Abstract:
Objective To observe the effects of G-CSF combined with SB203580(SB) on the immune system of mice received 4 Gy total body irradiation(TBI).
Methods Thirty male C57BL/6 mice were randomly divided into control group, irradiated group, combined group. The mice in irradiated and combined group received 4 Gy TBI. In combined group, G-CSF(1 μg/each) was intraperitoneally(ip) injected twice one day for 5 days, SB(15 mg/kg) was ip injected every other day after 4 Gy TBI for 5 times. After 4 Gy TBI 10 days, the peripheral bloods were counted, the CD4, CD8, B220 cells in WBC and CD4, CD8 in thymocytes were analyzed by flowcytometry. The reactive oxygen species levels(ROS) of bone marrow cells were detected by microplate reader.
Results Compared to the control group, the proportion of CD8, B220 and WBC in the irradiated mice significantly decreased, CD4⁺CD8⁺ thymocytes and ROS levels of bone marrow cells increased significantly. Compared to irradiation group, red blood, platelet, CD4 and CD8 cells in peripheral blood, CD4⁺CD8⁺ thymocytes decreased in the combined group.
Conclusion G-CSF combined with SB has protective effects on the radiation-induced injury in immune system.

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