DHEA对放、化疗损伤造血功能保护作用的实验研究

闫玉军; 叶翔; 宋娜玲; 王月英; 李德冠; 路璐; 孟爱民; 吴红英

doi:10.3760/cma.j.issn.1673-4114.2014.03.006

DHEA对放、化疗损伤造血功能保护作用的实验研究

Study on the protective function of DHEA on hematopoietic induced by radiotherapy and chemotherapy

摘要

摘要:
目的研究17aα-D-高炔雌二醇-3-乙酯（DHEA）对¹³⁷Cs γ射线和环磷酰胺（CTX）所致小鼠造血及免疫功能损伤的保护作用。
方法分别用8.0 Gy ¹³⁷Cs γ射线和CTX建立C57BL/6小鼠动物模型，观察DHEA低、中、高3个剂量对两个模型小鼠的造血干细胞脾结节形成细胞（CFU-S）数、外周血WBC、骨髓有核细胞数、脾脏指数的影响。
结果 DHEA各组对小鼠¹³⁷Cs γ射线照射所致的外周血WBC、CFU-S数、骨髓有核细胞数的降低具有保护作用，并增大了脾脏指数。DHEA各组对环磷酰胺引起的小鼠WBC、CFU-S数、骨髓有核细胞数降低有抑制作用。
结论 DHEA能减轻辐射和环磷酰胺对小鼠造血系统的损伤。

Abstract:
Objective To observe the protective function of dehydroepiandrosterone(DHEA) on hematopoietic in C57BL/6 mouse induced by radiotherapy and chemotherapy.
Methods Constructing the animal models with hematopoietic injury by 8.0 Gy ¹³⁷Cs γ ray and cyclophosphamide respectively. Observe the changes of colony-forming unit-spleen(CFU-S), populations of peripheral WBC and bone marrow nucleated cells and index of spleen in all DHEA treated groups.
Results DHEA can reduce injury of ¹³⁷Cs γ ray and cyclophosphamide induced hematopoietic function injury of C57BL/6 mouse. Compared with the blank control group, the DHEA group can significantly increase the number of peripheral blood leukocyte, bone marrow nucleated cells and CFU-S, and there is statistically difference between DHEA treated group and the blank control group. Index of spleen in the high dose group is higher than that in the blank control group.
Conclusion DHEA have protection function on hematopoietic in C57BL/6 mouse induced by radiotherapy and chemotherapy.

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