BTG2作为一种新的电离辐射诱导基因的研究

Study on B-cell translocation gene 2 as a novel radiation-induced gene

    摘要
  • 摘要:
    目的 研究γ射线照射对肿瘤细胞的B细胞易位基因2（BTG2）表达水平的影响。
    方法 应用RT-PCR方法研究电离辐射对肿瘤细胞BTG2 mRNA水平的影响；应用Western blot方法测定电离辐射对肿瘤细胞BTG2蛋白表达水平的影响。
    结果 与未照射对照细胞相比，3 Gy γ射线单次剂量照射明显提高了人乳腺癌细胞系MCF-7、MDA-MB-231和人宫颈癌细胞系C33A、HeLa中的BTG2蛋白的表达水平。γ射线照射人乳腺癌细胞系MCF-7和MDA-MB-231导致了照射剂量和时间依赖性的BTG2表达水平的升高，而且这种改变既表现在BTG2 mRNA水平上，也反映在BTG2蛋白水平上。
    结论 BTG2是一种新的辐射诱导DNA损伤的反应基因，进一步研究将为BTG2作为肿瘤放射治疗的疗效和预后评价指标提供实验基础和依据。

     

    Abstract:
    Objective To investigate the effects of ionizing radiation on the expression of B-cell translocation gene 2(BTG2) in cancer cells.
    Methods RT-PCR was used to determine the level of BTG2 mRNA, while the expression of BTG2 protein was assayed by Western blot.
    Results Compared with the un-irradiated cells, a single dose of 3 Gy γ-rays caused a significant increase of BTG2 protein in breast cancer cell lines, MCF-7 and MDA-MB-231, and cervical cancer cell lines, C33A and HeLa. Furthermore, irradiation with γ-rays resulted in a dose and time dependent increase of BTG2 at both mRNA and protein levels.
    Conclusion The present study demonstrates, for the first time, that BTG2 is a value response gene to exposure of ionizing radiation, which may provide evidence for BTG2 as a novel marker for radiotherapy of cancer.

     

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