抗辐射小鼠cDNA文库的全长cDNA功能鉴定

The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library

    摘要
  • 摘要:
    目的 鉴定IRM-2小鼠cDNA文库的全长cDNA功能。
    方法 以IRM-2小鼠的21条表达序列标签为引物进行PCR，从IRM-2小鼠全长cDNA文库中测定cDNA克隆的序列。观察小鼠胚胎成纤维细胞经6.5 Gy γ射线照射后全长cDNA的表达和全长cDNA转染对辐射敏感细胞共济失调性毛细血管扩张症（AT）5B1VA细胞生长的影响。
    结果 小鼠胚胎成纤维细胞经照射后，IRM-2小鼠4号、5号和2号cDNA表达水平均高于其亲本ICR小鼠和615小鼠。将全长cDNA转染AT5B1VA细胞，经照射后4号、5号和2号cDNA细胞存活率较高。
    结论 IRM-2小鼠4号、5号和2号全长cDNA具有较强的抗辐射功能。

     

    Abstract:
    Objective To identify the function of full-length cDNA sequence from IRM-2 mouse cDNA library.
    Methods Full-length cDNA products were amplified by PCR from IRM-2 mouse cDNA library according to twenty-one pieces of expressed sequence tag. The expression of full-length cDNAs were detected after mouse embryonic fibroblasts were exposed to 6.5 Gy γ-ray radiation. And the effect on the growth of radiosensitivity cells AT5B1VA transfected with full-length cDNAs was investigated.
    Results The expression of No.4, 5 and 2 full-length cDNAs from IRM-2 mouse were higher than that of parental ICR and 615 mouse after mouse embryonic fibroblasts irradiated with γ-ray radiation. And the survival rate of AT5B1VA cells transfected with No.4, 5 and 2 full-length cDNAs was high.
    Conclusion No.4, 5 and 2 full-length cDNAs of IRM-2 mouse are of high radioresistance.

     

    • HTML全文
    • 参考文献(13)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回