Abstract: ¹⁸F-FDG is currently the most widely used PET tracer. However, its application in the fields of brain tumor, prostate cancer, head and neck cancer, hepatocellular carcinoma, urinary tumor and well differentiated lung cancer exists limitation. Tumor uptake ¹¹C-acetate is based on accelerated lipid synthesis and impaired oxidative metabolism. ¹¹C-acetate PET may overcome the insufficiency of ¹⁸F-FDG PET. Uptake mechanism, application of the above tumors and the newest progress of ¹¹C-acetate PET and PET/CT are reviewed in this article.