角质细胞生长因子对受照小鼠的遗传保护作用

Keratinocyte growth factor pretreatment prevents radiation-induced genetics damage in a mouse model

    摘要
  • 摘要:
    目的 应用单细胞凝胶电泳（SCGE）和微核实验探讨角质细胞生长因子（KGF）对受照小鼠的遗传保护作用。
    方法 将40只C57BL/6J小鼠随机分为空白对照组、照射对照组、KGF低（1 mg/kg）、中（2 mg/kg）、高（4 mg/kg）剂量组5组。KGF的3个给药组于照射前给药，连续3 d分别给予腹腔注射1、2、4 mg/kg的KGF，1次/d。照射对照组腹腔注射磷酸盐缓冲液。给药后，空白对照组给予假照射，其他4组给予7.2 Gy 137Cs γ射线全身一次性照射。应用SCGE和微核实验检测5组小鼠的DNA损伤情况。
    结果 与照射对照组相比，KGF低剂量组小鼠的DNA微核率显著降低，中、高剂量组的彗星尾矩、Olive尾矩和微核率均显著降低。
    结论 KGF对受照小鼠有显著的遗传保护作用。

     

    Abstract:
    Objective To study the protective effect of keratinocyte growth factor (KGF) in genetics damage induced by radiation using the methods of single cell gel electrophoresis (SCGE) and micronuclei assay.
    Methods The C57BL/6J mice were randomly divided into five groups:control group, radiation group and low, middle, high dose groups of KGF(1, 2, 4 mg/kg). Drugs were administrated by intraperitoneal injection. Then control group was given sham-irradiated, and other groups were given a whole body irradiation at the dose of 7.2 Gy with γ-rays. DNA damage of different groups were detected by SCGE and micronuclei assay.
    Results Compared with radiation group, pretreatment with KGF can significantly decrease the tail moment and micronucleus formation.
    Conclusion Pretreatment with KGF can effectively protect the radiation-induced genetics damage in mouse model.

     

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