Tat-SmacN7蛋白对食管癌109细胞辐射敏感性的影响

The effects of Tat-SmacN7 protein on radiosensitivity of esophageal carcinoma l09

    摘要
  • 摘要:
    目的 探讨Tat-SmacN7蛋白（其中，Tat：反式激活蛋白；Smac：第二线粒体来源的caspase激活因子；N7：Smac的N端含有Ala-Val-Pro-Ile的七肽）对食管癌109（EC109）细胞放疗敏感性的影响。
    方法 对EC109细胞给予Tat-SamcN7、照射、以及联合处理，观察不同处理在24 h和48 h时对细胞的抑制作用，采用Western blot检测各组细胞中Smac蛋白表达水平。
    结果 通过水溶性四唑盐1检测发现Tat-SmacN7单独使用对细胞的抑制作用不明显，但与Tat-SmacN7组和照射组相比，Tat-SmacN7联合放射组可以明显增强对细胞的抑制（24 h：t=16.821和9.825，P < 0.05；48 h：t=23.553和11.930，P < 0.05）。说明Tat-SmacN7能增强EC109细胞对放射的敏感性。Western blot证明了Tat-SmacN7可以使细胞内Smac蛋白表达增加。
    结论 Tat-SmacN7蛋白能通过细胞高表达Smac，增强EC109细胞对辐射的敏感性。

     

    Abstract:
    Objective To investigate the effects of Tat-SmacN7 protein on sensitivity of esophageal carcinoma 109(EC109) to radiation.
    Methods Cells were treated with Tat-SmacN7 peptides, radiation or combination, the inhibition rate of EC109 cells were detected by water-soluble tetrazolium salt-1 assay. The protein level expression of Smac was determined by Western blot.
    Results The cells were resistant to Tat-SmacN7 as a single agent, but it could improve the sensitization of EC109 to radiation. Compared with the Tat-SamcN7 group and the radiation group, the Tat-SmacN7 combined with radiation group was inhibited significantly(24 h:t=16.821 and 9.825, both P < 0.05; 48 h:t=23.553 and 11.930, both P < 0.05). Western blot assay showed that Tat-SmacN7 could increase the expression of Smac protein.
    Conclusions Tat-SmacN7 could improve the radiosensitization of EC109 cell by increasing the expression of Smac.

     

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