电离辐射对原代成骨细胞M-CSF表达的影响

Effects of radiation on macrophage colony stimulating factor in primary osteoblasts

    摘要
  • 摘要:
    目的 研究辐射对于原代成骨细胞巨噬细胞集落刺激因子（M-CSF）表达的影响，进而研究辐射导致骨损伤的分子机理。
    方法 采用原代骨髓间充质干细胞诱导分化为成骨细胞，经0、2、4 Gy 137Cs γ射线照射后，采用实时定量PCR以及Western blot方法检测辐射对M-CSF mRNA和蛋白表达水平的影响。
    结果 2 Gy和4 Gy γ射线照射均能引起成骨细胞M-CSF mRNA（t=-17.329, P < 0.01；t=-3.841, P < 0.05）和蛋白表达水平上调。4 Gy照射后则会导致成骨前体细胞M-CSF mRNA表达水平上调（t=-4.478, P < 0.05），但与对照组比较，两者的蛋白表达水平未见显著差异。
    结论 2 Gy和4 Gy γ射线照射后，成骨细胞M-CSF表达水平上调能增强核因子κB受体活化因子配体对破骨细胞分化、成熟的促进作用，有助于增强破骨细胞的骨吸收能力。

     

    Abstract:
    Objective To investigate the radiation effect on the expression of macrophage colony stimulating factor(M-CSF)in primary osteoblasts and the molecular mechanism of bone injury induced by radiation.
    Methods Osteoblasts differentiated from bone marrow stromal cells(BMSCs)were analyzed by real-time PCR and Western blot after 2 Gy or 4 Gy 137Cs γ-irradiation.
    Results M-CSF mRNA and protein expression level were up-regulated after 2 Gy and 4 Gy irradiation(t=-17.329, P < 0.01; t=-3.841, P < 0.05)in osteoblasts. 4 Gy irradiation increased M-CSF mRNA in osteoblast precursors(t=-4.478, P < 0.05), but do not affect the protein expression level.
    Conclusions These results indicated that up-regulated M-CSF in osteoblasts could enhance the function of osteoclasts′ differentiation and maturation which induced by receptor activator of nuclear factor κB ligand after 2 Gy and 4 Gy irradiation. Further more, up-regulated M-CSF expression could enhance osteoclastic bone resorption of mature osteoclasts.

     

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