CD4+CD25+CD127-调节性T细胞在Graves病中的变化及临床意义

邹劲涛; 余佩玲; 董经纬; 廖启洪; 刘东亮; 曾鸿毅

doi:10.3760/cma.j.issn.1673-4114.2012.06.010

CD4⁺CD25⁺CD127^-调节性T细胞在Graves病中的变化及临床意义

Changes and clinical significance of CD4⁺CD25⁺CD127^- regulatory T cells in Graves disease

摘要

摘要:
目的通过观察Graves病患者外周血中CD4⁺CD25⁺CD127^-调节性T细胞(Treg)数量的变化, 探讨Graves病发生的免疫学机制。
方法采用流式细胞术检测90例Graves病患者和50名正常人外周血中CD4⁺CD25⁺CD127^-Treg的比例, 同时测定甲状腺功能指标及甲状腺自身抗体水平。采用t检验进行组间比较, 采用线性相关分析法分析CD4⁺CD25⁺CD127^- Treg与甲状腺功能指标的关系。
结果 90例Graves病患者与50名正常人外周血中CD4⁺CD25⁺CD127^-Treg的比例分别为1.39%±1.09%和4.59%±1.14%, 两者间差异有统计学意义(t=16.4, P＜0.01)。CD4⁺CD25⁺CD127^- Treg数量与促甲状腺激素受体抗体水平呈负相关(r=-0.24, P＜0.05), 与总三碘甲状腺原氨酸、总甲状腺素、游离三碘甲状腺原氨酸、游离甲状腺素、甲状腺球蛋白抗体、甲状腺微粒体抗体水平呈负相关(r=-0.62、-0.65、-0.56、-0.71、-0.50、-0.15, P均＜0.01)。
结论 Graves病患者CD4⁺CD25⁺CD127^-Treg数量减少, 且CD4⁺CD25⁺CD127^- Treg数量与甲状腺功能指标及甲状腺自身抗体水平关系密切, 提示CD4⁺CD25⁺CD127^-Treg数量减少可能与Graves病的发病有关。CD4⁺CD25⁺CD127^-可能是人CD4⁺ T细胞中Treg的特异性标志物。

Abstract:
Objective To investigate the mechanism of Graves disease by observing the changes of CD4⁺CD25⁺CD127^- regulatory T cells (Treg) population in the patients.
Methods Flow cytometry was used to detect the proportion of CD4⁺CD25⁺CD127^- Treg of CD4⁺ T cells in 90 Graves disease patients (Graves disease group) and 50 healthy adults(control group). Thyroid function and autoantibody levels were determined simultaneously. The t test was adopted for comparison between groups. The relationship between CD4⁺CD25⁺CD127^- Treg and thyroid function was analyzed by linear correlation analysis.
Results The percentages of CD4⁺CD25⁺CD127^-Treg in Graves disease group and control group were 1.39%±1.09% and 4.59%±1.14% separately. There was significant difference between the two groups(t=16.4, P < 0.01). There were negative correlation between CD4⁺CD25⁺CD127^- Treg percentages and total triiodothyronine, total thyroxine, free triiodothyronine, free thyroxine and thyrotropin receptor antibody, thyroglobulin antibody, thyroid microsomal antibody(r=-0.62, -0.65, -0.56, -0.71, -0.50, -0.15, all P < 0.01).
Conclusions The reduction of CD4⁺CD25⁺CD127^-Treg percentages in Graves disease group and close relations of CD4⁺CD25⁺CD127^-Treg with thyroid function and thyroid autoantibody levels suggest that CD4⁺CD25⁺CD127^-Treg decrease in the number may be associated with the onset of Graves disease. CD4⁺CD25⁺CD127^- may be the specific marker of Treg.

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CD4+CD25+CD127-调节性T细胞在Graves病中的变化及临床意义

Changes and clinical significance of CD4+CD25+CD127- regulatory T cells in Graves disease

CD4⁺CD25⁺CD127^-调节性T细胞在Graves病中的变化及临床意义

Changes and clinical significance of CD4⁺CD25⁺CD127^- regulatory T cells in Graves disease