小鼠不同肿瘤模型对放化疗敏感性的研究

The radiation sensitivities of different mice tumor model to radiotherapy and chemotherapy

    摘要
  • 摘要:
    目的 建立人食管癌及小鼠不同移植瘤模型，比较放、化疗对小鼠的不同肿瘤模型的抑制作用。
    方法 动物模型建立24 h后，将不同肿瘤细胞（淋巴瘤、肉瘤S180、肝癌H22、白血病L1210）的荷瘤IRM-2近交系小鼠随机分为对照组、照射组、环磷酰胺组，每组10只。环磷酰胺组于接种隔日腹腔注射环磷酰胺（25 mg/kg）1次，0.2 ml/只，共4次。照射组于接种后第4天进行全身1 Gy照射，每日1次，连续5 d。将食管癌EC901荷瘤裸鼠随机分为对照组、照射组、5-氟尿嘧啶组，每组5只。照射组于接种后第4天和第8天对肿瘤局部进行2 Gy照射，5-氟尿嘧啶组于接种后隔日腹腔注射5-氟尿嘧啶（25 mg/kg）1次，0.2 ml/只，共4次。所有小鼠于第12天处死，解剖瘤块称重，计算抑瘤率。
    结果 裸鼠及IRM-2近交系小鼠皮下移植瘤模型的成瘤率均为100%，放疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210荷瘤小鼠的肿瘤有显著的抑制作用，抑瘤率分别为34.57%、32.69%、31.31%和18.32%，与对照组比较差异有统计学意义（t=4.130、3.222、3.581和2.713，P分别为 < 0.01、 < 0.01、 < 0.01、 < 0.05）。放疗对食管癌EC901的抑瘤率为22.99%，但与对照组比较差异无统计学意义（t=1.235，P > 0.05）。化疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210、食管癌EC901荷瘤小鼠的抑瘤率分别为74.47%、72.59%、69.12%、77.53%和56.32%，与对照组比较差异有统计学意义（t=12.694、12.208、7.223、11.964和5.266，P均 < 0.01）。
    结论 放、化疗对小鼠的不同肿瘤模型均有抑制作用。由于小鼠的品系和瘤源不同，对放、化疗的敏感性也存在差异。

     

    Abstract:
    Objective To set up the models of human esophageal cancer in nude mice and the models of different xenograft tumors in IRM-2 mice, observe the effects of chemotherapy and radiotherapy on the tumors.
    Methods Twenty-four hours after setting up the models, all the mice carrying tumors including lymphoma, hepatic carcinoma H22, leukemia L1210 and sarcoma S180 were randomly divided into 3 groups: control group, radiotherapy group and cyclophosphamidum group, 10 mice per group. Mice in cyclophosphamidum group were injected cyclophosphamidum(25 mg/kg) by intraperitoneal 4 times every other day; mice in radiotherapy group were given total body irradiation at the fourth day and last for 5 days. Mice with human esophageal cancer were randomly divided into 3 groups: control group, radiotherapy group and 5-fluorouracil group, 5 mice per group. Mice in radiotherapy group were given local body irradiation at the fourth day and the eighth day. Mice in 5-fluorouracil group were injected 5-fluorouracil(25 mg/kg) by intraperitoneal 4 times every other day. All the mice were killed at the twelfth day and the rates of tumor inhibition were calculated.
    Results The tumor inhibitory rates of IRM-2 mice in radiotherapy group were 34.57%(lymphoma), 32.69%(sarcoma S180), 31.31%(hepatic carcinoma H22) and 18.32%(leukemia L1210)respectively, and there were significant difference compared with control group(t=4.130, 3.222, 3.581 and 2.713, P < 0.01, < 0.01, < 0.01 and < 0.05). The tumor inhibitory rates of nude mice with human esophageal cancer in radiotherapy group was 22.99%, but there was no significant difference compared with control group(t=1.235, P > 0.05). The tumor inhibitory rates in radiotherapy group were 74.47%(lymphoma), 72.59%(sarcoma S180), 69.12%(hepatic carcinoma H22), 77.53%(leukemia L1210) and 56.32%(human esophageal cancer), and there were significant difference compared with control group(t=12.694, 12.208, 7.223, 11.964 and 5.266, all P < 0.01).
    Conclusions There were inhibitory effects of chemotherapy and radiotherapy on all types of tumors. However, the efficiency depends on the mouse strains and the type of tumor.

     

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