SKP2表达对食管癌细胞辐射敏感性的影响

Effects of SKP2 expression on radio-sensitivity of esophageal carcinoma

    摘要
  • 摘要:
    目的 探讨S期激酶相关蛋白2（SKP2）高表达和低表达对食管癌细胞辐射敏感性的影响。
    方法 用Western-blotting方法筛选SKP2低表达和高表达的食管癌细胞系；构建SKP2正义表达载体p-pcDNATM 3.1/myc-His A-SKP2和RNA干扰载体SKP2 RNAi，分别转染SKP2低表达和高表达的食管癌细胞系，用5 Gy γ射线照射各组细胞，通过克隆形成实验检测细胞辐射敏感性的差异。
    结果 SKP2在4种食管癌细胞中的表达水平依次为KYSE510＞KYSE450＞EC9706＞KYSE150。用p-pcDNATM 3.1/myc-His A-SKP2表达载体转染KYSE150细胞后SKP2表达水平升高，5 Gy γ射线照射后细胞的克隆形成能力显著高于对照组（F=3.53，P＜0.01），表明SKP2高表达可以降低食管癌细胞的辐射敏感性。RNA干扰载体转染KYSE510细胞后SKP2表达水平降低，5 Gy γ射线照射后细胞的克隆形成能力显著低于对照组（F=5.23，P＜0.05），表明敲降SKP2表达可以增加食管癌细胞的辐射敏感性。
    结论 SKP2表达与食管癌细胞的辐射敏感性密切相关，具体机制还需要进一步研究。

     

    Abstract:
    Objective To explore the correlation of S-phase kinase-associated protein 2(SKP2) expression with radio-sensitivity of esophageal carcinoma.
    Methods Detecting the level of SKP2 in four different esophageal carcinoma cell lines using Western-blotting. Constructing p-pcDNATM 3.1/myc-His A-SKP2 expression and SKP2 RNAi vector and transfecting SKP2 low or high expression cell, respectively. Clone forming assay was used to detect the cell proliferation ability of different groups after 5 Gy γ-ray irradiation.
    Results The sequence of SKP2 expression level in four different esophageal carcinoma cell line was KYSE510 > KYSE450 > EC9706 > KYSE150. Compared with parent control cells, the clone forming ability of SKP2 high expression cells was significantly increased(F=3.53, P < 0.01), showing high SKP2 expression depression the radio-sensitivity of KYSE150 cell. In contrast, the clone forming ability of KYSE510 cell was significantly decreased after SKP2 knock-down(F=5.23, P < 0.05), showing low SKP2 expression promoting the radio-sensitivity of KYSE510 cell.
    Conclusion SKP2 expression was correlated with radio-sensitivity of esophageal carcinoma, but further study was needed to explore the molecular mechanism.

     

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