白细胞介素21基因联合不同剂量γ射线照射对乳腺癌细胞生长的影响

Effects of interleukin-21 gene combined with different doses of γ-ray radiation on growth of breast carcinoma cells

    摘要
  • 摘要:
    目的 研究人白细胞介素21基因重组腺病毒载体（Ad-IL-21）联合不同剂量γ射线照射对人乳腺癌细胞体外生长的抑制作用。
    方法 采用随机数字表法设立空白对照组、β-半乳糖苷酶基因重组腺病毒载体（Ad-LacZ）对照组、Ad- IL-21组、γ射线照射组和Ad- IL-21联合γ射线照射组（联合照射组），将Ad-IL-21于体外转染人乳腺癌MCF-7细胞，转染6 h后进行0~10 Gy 137Cs γ射线照射，用噻唑蓝法检测MCF-7细胞的生长抑制率。
    结果 Ad-IL-21转染MCF-7细胞后，MCF-7细胞生长受到的抑制效应显著高于Ad-lacZ组（F=26.34，P＜0.05），单独照射组和联合照射组的抑制率均显著高于Ad-IL-21组（F=23.51，F=27.55，P均＜0.05），随着照射剂量的增大，细胞抑制率逐渐增高。同一照射剂量中，联合照射组对MCF-7细胞的抑制作用均显著高于γ射线照射组，联合照射组抑制率最高，与Ad-IL-21组和γ射线照射组比较，差异具有统计学意义（F=35.68，F=38.67，P均＜0.05）。
    结论 IL-21基因联合γ射线照射对乳腺癌细胞的抑瘤作用具有协同效应，有效地抑制肿瘤细胞的生长。

     

    Abstract:
    Objective To study the effect of recombinant adenovirus vector carrying interleukin-21 gene(Ad-IL-21) combined with different doses of γ-ray radiation on the growth of breast neoplasms.
    Methods MCF-7 cells were transfected by Ad-IL-21 and exposed from 0 to 10 Gy 137Cs γ-ray 6 h after transfection. The cohorts were divided into 5 groups:blank control group, adenovirus vector containing β-galactosidase gene(Ad-LacZ) group, Ad-IL-21 group, γ-ray radiation group and Ad- IL-21 combined with radiation group. The inhibition rates of MCF-7 cells were detected by MTT assay.
    Results MCF-7 cells transfected with Ad- IL-21 were inhibited significantly greater than that of Ad-LacZ(F=26.34, P < 0.05). The inhibition rates of γ-ray radiation group and Ad-IL-21 combined with radiation group were significantly higher than that of Ad- IL-21 group(F=23.51, F=27.55, both P < 0.05)and the inhibition rate increased when γ-ray radiation doses increased. The inhibition rate of Ad- IL-21 combined with radiation group was higher than that of γ-ray radiation group at the same dose. The inhibition rate of Ad- IL-21 combined with radiation group was the highest, which was significantly higher than that of Ad- IL-21 group and γ-ray radiation group(F=35.68, F=38.67, both P < 0.05).
    Conclusion The recombinant Ad- IL-21 transfection combined with γ-ray radiation shows the synergism for the inhibition of the growth of MCF-7 cells.

     

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