Abstract: Valine(V) being substituted for by glutamate(E)at codon 600 in B type RAF gene(BRAF^V600E mutation)is the most common genetic mutation in papillary thyroid cancer. There have been many researches shown a strong association between BRAF^V600E mutation and the occurrence, progression and metastasis of papillary thyroid cancer. This article illustrates its clinical utility on the preoperative diagnosis, prognosis evaluation and inhibitor treatment of papillary thyroid cancer, so as to instruct the clinical management better.