电化学发光法与放射性受体分析法检测促甲状腺激素受体抗体的对比研究

Comparative study between electrochemiluminescence immunoassay and radioactive receptor assay in measurement of serum thyrotropin receptor antibody

    摘要
  • 摘要:
    目的 比较电化学发光（ECLIA）与放射性受体分析（RRA）两种方法测定血清促甲状腺激素受体抗体（TRAb）水平的差异，探讨ECLIA法检测TRAb的可行性与临床应用价值。
    方法 75例患者，根据临床表现及实验室检查分为格雷夫斯病（GD）症状组32例、GD缓解组23例、对照组20例，采用ECLIA和RRA两种方法分别检测各受检组血清TRAb水平。
    结果 ①GD症状组用ECLIA与RRA两种方法测定的TRAb值均显著高于其他两组（F=11.814, F=3.404, 均P < 0.05）。②两种检测方法阳性符合率为95.7%，阴性符合率为73%，总符合率为80%。对于GD症状组和对照组，两种方法的阳性率的差异都没有统计学意义（χ2=3.691, χ2=1.026, 均P > 0.05）；而在GD缓解组中，ECLIA法阳性检出率显著高于RRA法（χ2=5.440, P < 0.05）。③两种方法所测TRAb值有明显的相关性（r=0.705, P < 0.01），但ECLIA法的检测值低于RRA法（Z=-4.399，P < 0.01）。
    结论 与RRA法相比，ECLIA法具有全自动化、简便、省时、灵敏度高等优点，更适合于对临床GD患者TRAb水平的监测。

     

    Abstract:
    Objective To compare the difference between electrochemiluminescence immunoassay (ECLIA) and radioactive receptor assay(RRA)in measurement of serum thyrotropin receptor antibody(TRAb), and to explore the clinical value and feasibility of ECLIA in TRAb.
    Methods Seventy-five patients were selected with symptomatic Graves'disease(GD)group 32 cases, GD in remission group 23 cases and control group 20 cases. Both ECLIA and RRA were used for the measurement of serum TRAb in all the patients.
    Results ①The values of TRAb with ECLIA and RRA in group of symptomatic GD were higher than those in the other two groups (F=11.814, F=3.404, P < 0.05). ② The positive and negative coincidence rate between the two methods were 95.7% and 73%, respectively. There was no significant difference of the positive rate between the two methods in both groups of symptomatic GD and control ( χ2=3.691, χ2=1.026, P > 0.05), but great difference in group of GD in remission, in which it was higher with ECLIA than with RRA (χ2=5.440, P < 0.05). ③ There was a good correlation between the two methods(r=0.705, P < 0.01), while the value detected by ECLIA was lower than that by RRA (Z=-4.399, P < 0.01).
    Conclusion ECLIA has the advantages of simple, convenient, timesaving, high sensitivity, and complete automatization, which is more suitable for clinical monitoring of serum TRAb for the patients with GD.

     

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