Abstract: More and more researchers pay close attention to the treatment of cancer with ¹²⁵I seeds implantation because of the advantages of little surgical trauma, good curative effect and little radiation damage tonormal tissue.As the clinical studyon ¹²⁵I seeds killing cancer has been performed profoundly, the correlated experimental study in vitro was carried out gradually.This review will focus on the recent advances in the experimental study and model design on continuous low-dose-rate irradiation by ¹²⁵I seeds killing cancer cell in vitro.