Ad-Rb94联合γ射线照射对食管癌细胞生长的影响

Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γray on growth of esophageal carcinoma cells

    摘要
  • 摘要:
    目的 探讨人视网膜母细胞瘤94基因重组腺病毒载体（Ad-Rb94）联合γ射线对人食管癌细胞体外生长的抑制作用。
    方法 采用随机数字表法将培养细胞分为空白对照组、β-半乳糖苷酶基因重组腺病毒载体（Ad-LacZ）对照组、Ad-Rb94组、γ射线照射组和Ad-Rb94联合γ射线照射组（联合组），Ad-LacZ和Ad-Rb94于体外转染人食管癌EC109细胞系，转染6 h后进行4 Gy 137Cs γ射线照射，用噻唑蓝法检测细胞的生长抑制率。
    结果 Ad-Rb94组、γ射线照射组和联合组对EC109细胞生长均具有抑制作用，其中，联合组抑制率最高（40.30±4.2）%，明显高于Ad-Rb94组（18.3±0.4）%和γ射线照射组(27.40±2.9)%（χ2=7.91, χ2=5.82，P < 0.05）；γ射线照射组与Ad-Rb94组比较差异具有统计学意义（χ2=5.12, P < 0.05）。
    结论 Ad-Rb94联合γ射线照射对食管癌细胞的抑瘤作用具有协同效应，能有效地抑制肿瘤细胞的生长。

     

    Abstract:
    Objective To study the combined effect of exogenous recombinant adenovirus-mediated retinoblastoma gene 94(Ad-Rb94)combined with γ-ray on the growth of esophageal carcinoma cells.
    Methods Cell culture were randomly divided into 5 groups: control group, recombinant adenovirus vector containing βgalactosidase gene(Ad-LacZ)group, Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γray radiation group.EC109 cells were transfected by Ad-Rb94 and exposed to 4 Gy 137Cs γ-ray irradiation 6 hours after transfection.The inhibition rate of EC109 cells were detected by MTT assay.
    Results EC109 cellstransfected with Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γ-ray radiation group were all inhibited.The inhibition rate of Ad-Rb94 combined with γ-ray radiation group reached(40.30%±4.2%), significantly higher than Ad-Rb94 group(18.3%±0.4%)and γ-ray radiation group(27.40%±2.9%)(χ2=7.91, χ2= 5.82, P < 0.05).The difference of inhibition rate between γ-ray radiation group and Ad-Rb94 group was extremely significant(χ2=5.12, P < 0.05).
    Conclusion The recombinant Ad-Rb94 gene transfection combined with γ-ray shows the synergism forthe inhibition of the growth of EC109 cells.

     

    • HTML全文
    • 参考文献(6)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回