Abstract: Positron radiopharmaceuticals are mainly used for PET studies, which are used in the field of nuclear medicine as tracers in the diagnosis and treatment of many diseases.They have important position and fuction in the clinical diagnosis and treatment.Metabolism or biotransformation will happen when PET radiopharmaceuticals enter into the body.Understanding the metabolic fate of radio-pharmaceutical probes is essential for an accurate analysis and interpretation of positron emission tomography imaging.The recent research progress on PET radiopharmaceuticals metabolism was reviewed in this paper, including the metabolism characteristics, research methods, analytical techniques and so on.