Abstract: Various imaging modalities have been exploited to investigate the anatomic or functional dissemination of tissues in the body.However, no single imaging modality allows overall structural, functional, and molecular information as each imaging modality has its own unique strengths and weaknesses.The combination of two imaging modalities that investigates the strengths of different methods might offer the prospect of improved diagnostic abilities.As more and more dual-modality imaging system have become clinically adopted, significant progress has been made toward the creation of dual-modality imaging probes, which can be used as novel tools for future multimodality systems.These all-in-one probes take full advantage of two different imaging modalities and could provide comprehensive information for clinical diagnostics.This review discusses the advantages and challenges in developing dual-modality imaging probes.