淋巴瘤细胞株Raji摄取18F-氟脱氧葡萄糖和18F-氟脱氧胸腺嘧啶核苷的体外实验研究

In vitro study of uptaking 18F-fluorodeoxyglucose and 18-fluorodeoxythymidine in human lymphoma Raji cell

    摘要
  • 摘要: 目的 研究测定人淋巴瘤细胞株Raji摄取18F-氟脱氧葡萄糖(18F-FDG)和18F-氟脱氧胸腺嘧啶核苷(18F-FLT)的方法并比较两者的差异。方法 在不同条件下测定淋巴瘤细胞株Raji对18F-FDG和18F-FLT的细胞结合率:细胞数量1×105~1×107/瓶;18F-FDG和18F-FLT的放射性活度1.85~29.6 kBq;反应时间20~120min;葡萄糖浓度0~11.1mmol/L。结果 每瓶1×107个细胞、加入3.7 kBq 18F-FDG、葡萄糖浓度在0~2.78 mmol/L、作用100min,18F-FDG细胞结合率达到(50.42±1.07)%;每瓶1×107个细胞、加入3.7 kBq 18F-FLT、作用100min,18F-FLT细胞结合率达到(59.48±0.77)%;18F-FDG和18F-FLT的细胞结合率之间具有统计学差异(F=1192.805,P<0.001)。结论 Raji细胞与18F-FDG的结合率与细胞数量、作用时间及葡萄糖浓度有关,与18F-FDG的放射性活度无关;Raji细胞与18F-FLT的结合率与细胞数量和作用时间有关,与18F-FLT的放射性活度及葡萄糖浓度无关;同等条件下,Raji细胞对18F-FLT的细胞结合率高于18F-FDG。

     

    Abstract: Objective To investigate the binding characteristics of 18F-fluorodeoxyglucose(18F-FDG) and 18F-fluorodeoxythymidine(18F-FLT) with human lymphoma cell Raji and the differences between them.Methods The binding rates of 18F-FDG and 18F-FLT was measured under different conditions:1×105~107 cells,1.85~29.6 kBq 18F-FDG or 18F-FLT, 0~11.1 mmol/L glucose levels and 20~120 min to measure.Results The binding rate of 18F-FDG was(50.42±1.07) % at the condition of l×107 cells, 3.7 kBq 18F-FDG,0~2.78 mmol/L glucose levels, 100 min, while 18F-FLT was(59.48 ±0.77) % at the same conditions. The difference of binding rates between 18F-FDG and 18F-FLT was significant(F=1192.805, P<0.001).Conclusion The uptake of 18F-FDG and 18F-FLT in human lymphoma Raji cell is high, and at the same condition, the uptake rate of 18F-FLT is higher than 18F-FDG.

     

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