Abstract: Objective To study radiation dose effect on three primary colors color value in trac, id brilliant red B solution. Methods Prepare aqueous solution with different eoneentration traeid brilliant red B, irradiated by 0-2000 Gy ⁶⁰Coγ-ray. Get data from the solution by special instrument which can detect three primary eolors information. Analyze, manipulate, draw eurves for the experiment result. Further preserve the irradiated sample four weeks in room temperature environment and repeat the prnc, ess above. Results The experiment result show three primary colors color value in the aqueous solutions changes by difterent doses ⁶⁰Coγ-ray. The red color value are gradually decreasing as the radiation dose is adding, the grecn color value are gradually increasing at the same time. The red and grecn color value ehanges evidently and trends adversely. The blue color value little changed. The experimental results have little changes after the irradiated samples preserve four weeks in room temperature environment. Conclusions The experiment demonstrate that radiation dose can be studied based on three primary colors color value, may be it will have more study subject by this methods.