Abstract: Objective To compare the difference between ⁹⁹Tc^m-diethylenetriaminepentaacetic acid (⁹⁹Tc^m-DTPA) renal dynamic imaging and measurement of serum cystatin C in evaluation of glomerular filtration rate (GFR).Methods One hundred patients were selected at random, 38 cases with hy-pertension, 35with nephrosis, 10 with arteriosclerosis obliterans, 9 with diabetes and 8 with other diseases. Evaluation of patients'GFR was made separately by ⁹⁹Tc^m-DTPA renal dynamic imaging and measurement of serum cystatin C.Results No significant difference of GFR was found between the two methods (t=1.591, P>0.05),and linear correlation was showed as well (r=0.809, P<0.01). The imaging also revealed the function of individaul kidney, renal blood perfusion and excretory function besides GFR.Conclusion s Results of GFR from the two methods revealed no significant difference and they were highly correlated. ⁹⁹Tc^m-DTPA renal dynamic imaging could reveal the individual renal function and provide a more comprehensive evaluation of renal function while the measurement of serum cystatin C was simple and convenient, timesaving, economic, and more suit-able for screening patients'renal function clinically.