Abstract: As one of the most important tracer for PET-CT, the preparation of ¹⁸F-FDG by nucleophilic substitution is better than electrophilic substitution,therefore the substitution of the former for the latter is imperative. Automatic synthesis module technology has powerful function on the large-scale production of radioactive drugs and widely clinical been used. In recent years the application of new technology in ¹⁸F-FDG synthesis module is very fascinating. The requirement for quality control of ¹⁸F-FDG is tremendously strict, for which the administration way of ¹⁸F-FDG is intravenous injection. The advanced detective methods and the standardization of Good Manufacturing Practice are guarantee to ¹⁸F-FDG quality control. The researches on pharmacokinetics of ¹⁸F-FDG show that the metabolic process accords with three-compartment model after intravenous, which provides a basis for clinical medication. In this paper, we review the ¹⁸F-FDG in detail from the principle of synthesis, craft process, quality control, metabolic distribution and clinical application and so on.