Abstract: Glomerular filtration rate (GFR) is used to evaluate renal function. In clinical practice, the accurate measurement of GFR is crucial for diagnosing and monitoring the progression of kidney diseases. Radionuclide tracer technology, a noninvasive and high-precision method, is widely used for the determination of GFR. In addition, radionuclide tracer technology can provide kidney morphological information, which is helpful for assessing functional differences between two kidneys. The authors outline GFR measurement methods based on radionuclide tracer technology to meet clinical requirements.