Abstract: ¹⁸F-fluorodeoxyglucose (FDG) micro-PET brain imaging is an important means of basic research in brain science. In animal experiments, brain activity is affected by experimental conditions such as diet, anesthesia, administration route of imaging agents, temperature and light. As a result, there will be deviation in cerebral perfusion and metabolic imaging, which will affect the evaluation of brain cognitive function. The authors review the effects of different experimental conditions on the brain uptake of ¹⁸F-FDG in rodent imaging studies, and provide guidance for animal imaging research.